Fig. 5

Inhibition of JAK2 suppresses the AS serum-mediated increase in ALP activity. a 293 T cells were transfected with ALP-luciferase plasmids and treated with various volumes of AS serum for 24 h, after which luciferase activity was analyzed. Representative data are shown (n = 3). b Ct-BdCs were transfected with ALP-luciferase plasmids and then stimulated for 24 h with serum from six patients with AS, with or without AG490. Ct-BdCs and AS-BdCs were stimulated with or without AG490 in the presence of OM for the indicated number of days. c The inhibitory effects of AG490 on Ct-BdCs and AS-BdCs were analyzed by assessing intracellular ALP activity over time. Representative data are shown (n = 4). d The inhibitory effects of AG490 on AS serum (1/10 dilution)-stimulated Ct-BdCs and AS-BdCs were analyzed by measuring intracellular ALP activity at early (left) and late (right) time points. Representative data are shown (n = 3). e The inhibitory effects of AG490 on Ct-BdCs and AS-BdCs were assessed by stimulating cells for 3 days and then analyzing the levels of the indicated proteins by immunoblotting. Representative data are shown (n = 5). One-way ANOVA analysis was performed to determine statistical significance. Data are presented as means ± SDs. *P < 0.05 indicates significant differences between the two groups