Fig. 3

Comparison of M1 and M2 macrophages (Mφ) in Behçet’s disease (BD) and systemic sclerosis (SSc) Mφ, and immunofluorescence assay of dermal tissue of a patient with BD. a IHC staining of erythema nodosum from patients with BD and sclerotic dermis of patients with SSc. Antibodies against CD163 and CCR1 (original magnification × 100 [H&E staining] and × 400 [IHC staining]) were used. b The ratio of CD163⁺ cells (defined as M2 Mφ) and CD68⁺ cells (defined as whole Mφ) were independently counted in three high-power fields, and the mean value for each patient was calculated. c Immunofluorescence analysis of CCR1 (green) and CD163 (red) in BD erythema nodosum tissue. Nuclei are stained with 4′,6-diamidino-2-phenylindole (DAPI; blue) (original magnification × 400). Arrowhead indicates CCR1 and CD163 double-positive cells (merged image)