Fig. 2

CircIBTK served as a miRNA sponge for miR-29b. a qRT-PCR analysis of nuclear and cytoplasmic RNAs showed that circIBTK was preferentially localized within the cytoplasm. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. b Predicted binding sites and corresponding mutant sites of circIBTK and miR-29b (wt, wild-type; mut, mutant type). c, d Effects of miR-29b on the expression of luciferase reporter genes containing circIBTK wt/mt binding site. Luciferase activity was normalized to the value obtained in the cells transfected with NC oligonucleotides. Results were represented as mean ± SD (n = 3). *P < 0.05, **P < 0.01. Con, control