Fig. 1
From: Methods for high-dimensional analysis of cells dissociated from cryopreserved synovial tissue
AMP consortium pipeline for high-dimensional synovial tissue assays. a Numerous Clinical Collection Sites contributed cryopreserved synovial samples to the Central Processing Site. b At the Clinical Collection Sites, synovial tissue was excised during research-oriented biopsies or routine removal in arthroplasty surgeries. Tissues and dissociated synovial cells cryopreserved for centralized processing. c Synovial tissue mechanically disaggregated via magnetic bar stirring in heated water bath for smaller biopsy fragments or gentleMACS dissociation for larger arthroplasty specimens. Tissue dissociation also involved incubation with protein and nucleic acid degrading enzymes at 37 °C. After filtration of debris, sample aliquots processed in parallel for mass cytometry (CyTOF) analysis and FACS-based sorting of four cell types for single-cell and bulk RNA sequencing (RNA-seq). AMP Accelerating Medicines Partnership