Fig. 3
From: Methods for high-dimensional analysis of cells dissociated from cryopreserved synovial tissue
Cryopreservation of synovial fragments retains stromal and hematopoietic cell viability and RA synovial gene expression patterns. a Frequency of synovial cell subpopulations from arthroplasty samples dissociated from freshly isolated or cryopreserved aliquots. b Cell viability frequency assayed by flow cytometry for synovial biopsy samples dissociated from fresh or cryopreserved tissue fragments. c Flow cytometry analysis of cells dissociated from synovial tissue either immediately or after cryopreservation. d Top 20 GO terms enriched for genes upregulated in RA patient disaggregated synovial cells in comparison to patients with OA. N = 10 RA and 10 OA samples with 2–8 replicates per donor. ns no significant difference, OA osteoarthritis, RA rheumatoid arthritis