Fig. 5
From: Methods for high-dimensional analysis of cells dissociated from cryopreserved synovial tissue
Low-input RNA-seq distinguishes cell populations sorted from dissociated RA synovial cells. a Flow cytometric detection of distinct cell populations obtained from cryopreserved RA synovial biopsy. b Number of cells of each targeted cell populations sorted from three different RA patient synovial biopsy (Bx) samples (two obtained by needle biopsy and one by portal and forceps biopsy from knee joint) for low-input RNA-seq. c Number of genes detected by low-input RNA-seq for each cell population sorted from same biopsies as (b). d Principal components analysis of sorted cell populations from three RA synovial biopsy samples based on 5006 genes with greatest variance. Color indicates sorted cell type, while shape indicates individual donor. FSC forward scatter, PC principal component, PI propidium iodide, SSC side scatter