Fig. 4

Batf KO inhibits CIA-induced bone erosion and cartilage destruction. a Representative images of pannus subjected to H&E and Safranin-O staining in NI and CIA WT and Batf^−/− DBA/1 J mice (left). Scoring of pannus formation (right; n = 9). b Representative images (n = 9) of receptor activator of NF-κB ligand (RANKL) immunostaining in ankle synovia of nonimmunized (NI) and collagen-induced arthritis (CIA) wild-type (WT) and Batf^−/− (knockout (KO)) DBA/1 J mice. c Tartase-resistant acid phosphatase (TRAP) staining and counting of TRAP-positive multinucleated cells (n = 6 mice per group) at the pannus-bone interface in ankle joints of NI and CIA WT and Batf^−/− DBA/1 J mice. d Cartilage destruction was detected by Safranin-O staining in NI and CIA WT and Batf^−/− DBA/1 J mice (left) and scored by OARSI grading (right; n = 12). Values are means ± SEM. Scale bars = 50 μm. b bone, c cartilage, p pannus