Fig. 1

CCL5 and CCR5 expression are increased in K/B.g7 mice. a Serum was collected from 8-week-old control nonarthritic B.g7 mice or arthritic K/B.g7 mice (n = 3/group). The concentration of CCL5 was determined by ELISA and data were compared using a Mann Whitney U test. Points represent individual mice; bars indicate mean ± SEM; *p < 0.05. b Sections of ankle joints from nonarthritic control mice or K/B.g7 mice were stained with isotype control antibody or anti-CCR5 antibody, as indicated. Brown indicates positive staining. The black box in the lower-power (4× objective) images indicates the region of the tibiotalar joint magnified (20× objective) in the right images. Relative to the control ankles, ankles from the arthritic mice have joint space narrowing, cartilage loss, and numerous CCR5-expressing inflammatory cells