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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cells

Fig. 1

Identification of adipose-derived mesenchymal stem cells (ASCs). a-d Multilineage differentiation ability of the ASCs, verified by histological assays. a Oil Red O staining showed the adipogenic differentiation potential of the ASCs. The red dots (indicated by arrows) stand for the differentiated adipocytes colored by Oil Red O staining. b Alcian Blue staining for chondrogenic differentiation. The blue deposits were indicative of functional chondrocytes. The osteogenic differentiation verified by Alizarin Red (c), the calcium deposits were stained red (indicated by arrows) and the osteoblasts were in dark blue stained by alkaline phosphatase staining (d). e Expression of specific surface markers of ASCs by flow cytometry. The ASCs positively expressed CD90, CD105 and HLA-ABC and negatively expressed CD34, CD45 and HLA-DR

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Arthritis Research & Therapy

ISSN: 1478-6362

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