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Table 1 Primers used in real-time PCR

From: Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cells

Gene name

Forward sequence 5′-3′

Reverse sequence 5′-3’

PIK3R3

GGG GAA GTG AAG CAC TGT GT

GAC GTT GAG GGA GTC GTT GT

ENPP1

GCC CGA AAT CTT TCT TGC CG

TGC CAT GCT TGA ATC CAG GT

MT1F

TGC AAG TGC AAA GAG TGC AA

CCC TTT GCA AAC ACA GCC C

SPP1

GCC GAG GTG ATA GTG TGG TT

AAC GGG GAT GGC CTT GTA TG

EPYC

TTC TGG GGC CAC ACA CAA AT

GCT CTC GAA GTT GAG GCA GT

CD24

GCT CCT ACC CAC GCA GAT TT

GAG ACC ACG AAG AGA CTG GC

C4orf31

TCA TGT CTA CTC CAG GCC CA

GTA GTA CTG CGT GTC GGG TT

homo-actin

GCT CAG GAG GAG CAA TGA TCT TG

GTA CGC CAA CAC AGT GCT GTC

  1. PIK3R3 phosphoinositide-3-kinase, regulatory subunit 3, ENPP1 ectonucleotide pyrophosphatase/ phosphodiesterase 1, MT1F metallothionein 1F, SPP1 secreted phosphoprotein 1, EPYC epiphycan, C4orf31 chromosome 4 open reading frame 31
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Arthritis Research & Therapy

ISSN: 1478-6362

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