Skip to main content

Table 1 Primers used in real-time PCR

From: Aberrantly expressed messenger RNAs and long noncoding RNAs in degenerative nucleus pulposus cells co-cultured with adipose-derived mesenchymal stem cells

Gene name Forward sequence 5′-3′ Reverse sequence 5′-3’
PIK3R3 GGG GAA GTG AAG CAC TGT GT GAC GTT GAG GGA GTC GTT GT
ENPP1 GCC CGA AAT CTT TCT TGC CG TGC CAT GCT TGA ATC CAG GT
MT1F TGC AAG TGC AAA GAG TGC AA CCC TTT GCA AAC ACA GCC C
SPP1 GCC GAG GTG ATA GTG TGG TT AAC GGG GAT GGC CTT GTA TG
EPYC TTC TGG GGC CAC ACA CAA AT GCT CTC GAA GTT GAG GCA GT
CD24 GCT CCT ACC CAC GCA GAT TT GAG ACC ACG AAG AGA CTG GC
C4orf31 TCA TGT CTA CTC CAG GCC CA GTA GTA CTG CGT GTC GGG TT
homo-actin GCT CAG GAG GAG CAA TGA TCT TG GTA CGC CAA CAC AGT GCT GTC
  1. PIK3R3 phosphoinositide-3-kinase, regulatory subunit 3, ENPP1 ectonucleotide pyrophosphatase/ phosphodiesterase 1, MT1F metallothionein 1F, SPP1 secreted phosphoprotein 1, EPYC epiphycan, C4orf31 chromosome 4 open reading frame 31