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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Evaluation of 99mTc-rhAnnexin V-128 SPECT/CT as a diagnostic tool for early stages of interstitial lung disease associated with systemic sclerosis

Fig. 2

Identification of cell types undergoing apoptosis in the model of bleomycin (BLM)-induced lung fibrosis. Immunohistochemical co-staining of cleaved caspase 3 (brown) with the epithelial cell marker E-cadherin (green) (a), with the endothelial cell marker von Willebrand factor (vWF) (green) (b), with the pan-leucocyte marker CD45 (green) (c) and with the smooth muscle cell and myofibroblast marker alpha smooth muscle actin (αSMA) (red) (d). Magnification is × 400. Inlets represent zoomed images. Representative pictures from three mice each are shown. Scale bars 20 μm. Red arrows highlight double staining with the cell-type-specific markers, black arrows show single stained apoptotic cells

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