Fig. 4

Identification of cell types undergoing apoptosis in the fos-related antigen 2 (Fra-2) transgenic (tg) mouse model. Immunohistochemical co-staining of cleaved caspase 3 (brown) with the epithelial cell marker E-cadherin (green) (a), with the endothelial cell marker von Willebrand factor (vWF) (green) (b), with the pan-leucocyte marker CD45 (green) (c), and with the smooth muscle cell and myofibroblast marker alpha smooth muscle actin (αSMA) (red) (d). Magnification is × 400. Inlets represent zoomed images. Representative pictures from three mice each are shown. Scale bars 20 μm. Red arrows highlight double staining with the cell-type-specific markers, black arrows show single stained apoptotic cells