Fig. 5

Imaging of apoptotic cells with ^99mTc-rhAnnexin V-128 in bleomycin (BLM)-treated mice. a Biodistribution of ^99mTc-rhAnnexin V-128 in relevant organs of BLM-treated mice and saline controls. b Significantly greater lung uptake of the ^99mTc-rhAnnexin V-128 radiotracer (4 h post injection (p.i.)) in the lungs of BLM-treated mice at day 3 after the BLM instillation. c Ex vivo autoradiography of frozen lung sections derived from BLM-treated mice and controls showing higher accumulation of ^99mTc-rhAnnexin V-128 (4 h p.i.) in BLM-treated mice versus controls at day 3 post instillation. d In vivo single photon emission computed tomography (SPECT/CT) of ^99mTc-rhAnnexin V-128 (4 h p.i.) administrated to BLM-treated mice and saline controls at day 3 post instillation. Herein, the chest cavity including the heart and the lungs is shown. Data are expressed as mean ± SD, n = 3, *p < 0.05, unpaired parametric Student’s t test. % IA/g, percentage of injected activity per gram tissue