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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Loss of Gαq impairs regulatory B-cell function

Fig. 2

Gαq deficiency did not affect TLR4 signaling in B cells. a, b B cells purified from WT and Gnaq−/− mice stimulated with LPS for 48 h, and PMA, ionomycin, and BFA added for last 5 h. Fixable Viability Dye eFlour™ 510 used to analyze B-cell death by flow cytometry. Data presented as mean ± SD of five mice. Results shown are one from three independent experiments. Student’s t test analyzed statistical difference. ***p < 0.001. c Splenic cells from WT and Gnaq−/− mice stained with anti-mouse CD19 and anti-mouse TLR4, followed by flow cytometry analysis (n = 5). Representative histogram shown. Blue line, WT mice; red line, Gnaq−/− mice. Data representative of three independent experiments. d WT and Gnaq−/− B cells treated with LPS, then harvested to analyze Myd88 expression by western blot analysis at indicated times. Data representative of three independent experiments. Breg regulatory B cell, IL interleukin, LPS lipopolysaccharide, MyD88 myeloid differentiation primary response 88, TLR Toll-like receptor

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