Skip to main content
Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Loss of Gαq impairs regulatory B-cell function

Fig. 4

Loss of Gαq in Bregs reduces Tregs in vitro. CD19+CD1dhiCD5+ Bregs isolated from WT or Gnaq−/− mice and cocultured with purified CD4+CD25 T cells from spleen of WT mice for 72 h in presence of Mouse T-Activator CD3/CD28 Dynabeads™. After 72 h, cells and culture supernatants harvested. a, b Cells stained with anti-CD4, anti-CD25, and anti-Foxp3 antibody to analyze Tregs. c Supernatants harvested for ELISA analysis of IL-6 and TGF-β. Results represent mean ± SD per group (n = 5 mice/group). Data representative of three independent experiments. Student’s t test analyzed statistical difference. *p < 0.05, ***p < 0.001. Breg regulatory B cell, IL interleukin, TGF-β transforming growth factor beta, Treg regulatory T cell

Back to article page