Fig. 12

Apoptosis-associated speck-like protein containing CARD (ASC) phosphorylation in granulocyte–macrophage colony-stimulating factor (GM-CSF)-treated neutrophils. Neutrophils were incubated with GM-CSF (10 ng/mL) in the presence or absence of tofacitinib for 8 h. Cellular lysates were subjected to Western blot using anti-phospho-ASC (Tyr-144) or anti-β-actin antibodies. Data are representative of two independent experiments. Abbreviation: MW molecular weight