Fig. 3
Tofacitinib inhibits the caspase-1 (p20) release from granulocyte–macrophage colony-stimulating factor (GM-CSF)-stimulated neutrophils. Neutrophils (2 × 106/mL) were stimulated with GM-CSF (10 ng/mL) in the presence or absence of the indicated concentrations of tofacitinib for 24 h, and supernatants were analyzed for caspase-1 (p20) by enzyme-linked immunosorbent assay. Values represent the mean ± standard deviation of two independent experiments. *P <0.01 compared with GM-CSF–stimulated neutrophils. Abbreviation: TNF-α tumor necrosis factor-alpha