Fig. 7

Phosphorylation of nuclear factor-kappa B (NF-κB) p65 in granulocyte–macrophage colony-stimulating factor (GM-CSF)-treated neutrophils. Neutrophils were stimulated with GM-CSF (10 ng/mL) for 20 min. Cells were lysed and cellular lysates were subjected to Western blot using anti-NF-κB, phosphor-NF-κB, IκB-α, phosphor-1κB-α, and β-actin antibodies. Data are representative of two independent experiments