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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: An explorative study on deep profiling of peripheral leukocytes to identify predictors for responsiveness to anti-tumour necrosis factor alpha therapies in ankylosing spondylitis: natural killer cells in focus

Fig. 2

Representative gating strategy to identify CD8-positive and CD8-negative NK cell subsets. To increase parameter diversity detectable in a single seven-colour staining setup we have combined specifically expressed cell lineage markers that are labelled by the same fluorochrome, such as CD14-PE/CD56-PE/IgD-PE. Its cell-specific expression can be deconvoluted by sequential gating as exemplified by a: (left panel) gating on small cells (G1) with low granularity according to FSC/SSC characteristics allows exclusion of monocytes (CD14) and granulocytes (CD16); (middle panel) exclusion of B lymphocytes by CD19 and T lymphocytes by CD3 (G2); and (right panel) CD19/CD3-double negative cells were analysed for CD56 versus CD16 allowing us to identify NK cells by the co-expression of CD56 and CD16 (G3). b Classical NK cells were further analysed according to their expression of the CD8 receptor in five exemplary responders (R; top row) and non-responders (NR; bottom row)

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