Fig. 7

Impact of recombinant human proteoglycan-4 (rhPRG4) treatment on differential weight bearing (Right hind limb – Left hind limb; R-L) and synovial fluid (SF) lavage myeloperoxidase (MPO) activity following intra-articular administration of monosodium urate monohydrate (MSU) crystals (50μL; 2.5 mg/mL) in the right knee joint of male Lewis rats followed by intra-articular treatments with rhPRG4 (1 mg/mL; 50 μL) or PBS (50 μL) at 1 h following MSU administration, or no treatment. Differential weight bearing was measured at 3 h (n = 12 in MSU alone and n = 14 in rhPRG4 or PBS treatments), 6 h (n = 12 in MSU alone and n = 14 in rhPRG4 or PBS treatments) and 24 h (n = 5 in MSU alone and n = 7 in rhPRG4 or PBS treatments) following MSU administration. SF lavage MPO activities were determined at 6 h (n = 7 in each group) and 24 h (n = 5 in each group) following MSU administration. Data are presented as a scatterplot with the mean value highlighted. *p < 0.001; **p < 0.01; ***p < 0.05. a rhPRG4 treatment decreased MSU-induced differential weight bearing at 6 h compared to PBS treatment or no treatment. b rhPRG4 treatment reduced MSU-induced elevation in SF lavage MPO activity at 6 h compared to PBS treatment or no treatment