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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Osteoporosis of the vertebra and osteochondral remodeling of the endplate causes intervertebral disc degeneration in ovariectomized mice

Fig. 5

Representative images of safranin O and fast green staining of the intervertebral disc (IVD). This showed consistent results with H&E staining. Specially, thickening of the bony endplate (EP; double arrow) accompanied by accelerated osteochondral remodeling (black arrows) and duplication of tidemarks (yellow arrow) were displayed more clearly in the EP of ovariectomized (OVX) mice by safranin O fast green staining. Moreover, reduction of aggrecan in the nucleus pulposus (NP), as indicated by a paucity of safranin O staining (blue thick arrow), cleft/crack formation in the annulus fibrosus (AF) (blue arrow), and loss of cells indicate intervertebral disc degeneration in OVX mice. n = 9 per group; scale bars = 100 μm and 20 μm as indicated

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Arthritis Research & Therapy

ISSN: 1478-6362

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