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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Osteogenic differentiation of fibroblast-like synovial cells in rheumatoid arthritis is induced by microRNA-218 through a ROBO/Slit pathway

Fig. 4

Influence of overexpression and knockdown of microRNA-218-5p (miR-218) on the expression of roundabout1 (ROBO1). Expression of ROBO1 in rheumatoid arthritis (RA)-fibroblast-like synovial cells (FLS) was determined relative to the controls transfected with scrambled RNA, which was defined as 1. a Transfection of RA-FLS (n = 5) with precursor miR-218 (pre-miR-218) for 48 h decreased the levels of ROBO1 compared to scrambled RNA-transfected controls, as determined by SYBR green real-time PCR analysis. b Knockdown of miR-218 for 48 h in RA-FLS (n = 5) increased the level of ROBO1 compared to scrambled RNA-transfected controls, as determined by SYBR green real-time PCR analysis. Values are presented as means ± SEM: *p < 0.05 versus scrambled RNA-transfected controls, as determined by Student’s paired t test

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