Table 1 SYBR green primers used for real-time PCR
ALP forward | 5′-AGCTCAACACCAACGTGGCTAA-3′ |
ALP reverse | 5′-TTGTCCATCTCCAGCCTGGTC-3′ |
RUNX2 forward | 5′-CTTTGTAGCACAAACATTGCTGGA-3′ |
RUNX2 reverse | 5′-AAAGCTGTGGTACCTGTTCTGGA-3′ |
CTNNB1 forward | 5′-CATCCTAGCTCGGGATGTTCAC-3′ |
CTNNB1 reverse | 5′-TCCTTGTCCTGAGCAAGTTCAC-3′ |
CDH11 forward | 5′-CAGGTGCTACAGCGCTCCAA-3′ |
CDH11 reverse | 5′-TTAATGTTCCCATCACCAGAGTCAA-3′ |
ROBO1 forward | 5′-CGGCAGAGTATGCTGGTCTGAA-3′ |
ROBO1 reverse | 5′-CTAGGGCACTGAGACGCATGAA-3′ |
DKK1 forward | 5′-CCAGACCATTGACAACTACCAG-3′ |
DKK1 reverse | 5′-AGGCGAGACAGATTTGCAC-3′ |
GAPDH forward | 5′-GCACCGTCAAGGCTGAGAAC-3′ |
GAPDH reverse | 5′-TGGTGAAGACGCCAGTGGA-3′ |