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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state

Fig. 1

The direct effects of MSU crystals on osteocyte viability. The alamarBlue® assay was used to determine the viability of a MLO-Y4 cells and primary mouse osteocytes cultured with monosodium urate (MSU) crystals for 24 h, b MLO-Y4 cells cultured with soluble urate for 24 h, and c MLO-Y4 cells cultured with different types of crystals for 24 h. Viability was assessed 24 and 48 h after the addition of crystals or soluble urate. Data shown are pooled from three to four biological repeats and are presented as mean (SEM); by two-way ANOVA a PInteraction < 0.0001 for MLO-Y4 cells, PInteraction = 0.026 for primary mouse osteocytes, b PInteraction = 0.24, and c PInteraction = 0.057 at 24 h, PInteraction < 0.0001 at 48 h; with post-hoc Dunnett’s test *p < 0.05, **p < 0.01, and ***p < 0.001 versus control (no crystals or soluble urate) at that time point. d The LIVE/DEAD® assay was used to determine the percentage of dead MLO-Y4 cells within three separate layers of the collagen gel following culture with MSU crystals for 24 h or 48 h. Data shown are pooled from four biological repeats and are presented as mean (SEM); one-way ANOVA p < 0.0001 at 24 h, p = 0.004 at 48 h; with post-hoc Sidak’s test ***p < 0.001 versus control (no MSU crystals) for each layer of the gel. BCP basic calcium phosphate, CPPD calcium pyrophosphate dehydrate

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