Fig. 2
Direct effects of MSU crystals on MLO-Y4 cell expression of bone-related or inflammatory genes. Real-time PCR was used to determine changes in the relative mRNA expression levels of a bone-related and b inflammatory genes in MLO-Y4 cells, following culture with 0.1 mg/mL monosodium urate (MSU) crystals for 0, 1, 6, and 24 h. Data shown are pooled from three biological repeats and are presented as mean (SEM); two-way ANOVA PInteraction > 0.1 for all genes. OPG osteoprotegerin, RANKL receptor activator of nuclear factor kappa-B ligand, TNF tumor necrosis factor