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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA

Fig. 4

Effect of PECAM-1 on degranulation in CD177-negative neutrophils. a SHP-1 phosphorylation was detected in neutrophils incubated with platelet endothelial cell adhesion molecule-1 (PECAM-1). b Effect of PECAM-1 on degranulation of CD177-negative and mixed neutrophils. Degranulation induced by proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin (Ig)Gs (PR3 ANCA) was little influenced by preincubation with soluble PECAM-1 in CD177-negative neutrophils, but it was significantly inhibited by soluble PECAM-1 in mixed neutrophils. The level of lactoferrin was expressed as a percentage of control in each subset. The purity of the CD177-positive subset in mixed neutrophils was 72.7 ± 10.7% and the purity of CD177-negative neutrophils after selecting was 86.4 ± 8.5%. Bars represent mean ± SD of repeated measurements from four independent experiments. **p < 0.01, ***p < 0.001. MW molecular weight, PMA phorbol myristate acetate, TNF-α tumor necrosis factor-alpha

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Arthritis Research & Therapy

ISSN: 1478-6362

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