Fig. 6

STAT1/MyD88 complexes mediate E2F2 regulation of inflammatory cytokines. a–d STAT1/MyD88 complex is found in RASFs and MEFs. E2F2-silenced RASFs and E2f2^−/− MEFs were cultured with or without lipopolysaccharide (LPS; 10 μg/mL). Co-IP (a,b) was performed to test the binding. Confocal immunofluorescence (c,d) was performed to confirm the result (magnification 10 × 40, MyD88 (green) and STAT1 (red)). e–g Effect of STAT1/MyD88 complexes on the expression of cytokines. qRT-PCR was performed to detect expression of interleukin (IL)-1α (e), IL-1β (f), and tumor necrosis factor (TNF)-α (g) in STAT1/MyD88 knockdown RASFs with or without E2F2 overexpression in the presence of LPS (10 μg/mL). The results shown are means ± SEM of three independent experiments performed in triplicate. **P < 0.01, ***P < 0.001, versus the control. h Model depicting the role of E2F2 in RA pathogenesis. Ad-GFP adenovirus encoding green fluorescent protein, NC knockdown scramble control, si small interfering, WT wild-type