Table 1 Primers used for real-time polymerase chain reaction
Primer name | Primer base sequence (5’ to 3’) |
|---|---|
GAPDH (homo) | Forward: CACCATCT TCCAGGAGC;Reverse: AGTGGACTCCACGACGTA |
GAPDH (mus) | Forward: AAAGGGTCATCATCTCCG;Reverse: CAATCTTGAGTGAGTTGTCATATTTC |
E2F2 (homo) | Forward: CCTTGGA GGCTACTGACAGC;Reverse: CCACAGGTAGTCGTCCTGGT |
E2F2 (mus) | Forward: TGTTTCCCTGGGAGGATTATT;Reverse: TTTGGGACAGTGGGTGTTTA |
STAT1 (homo) | Forward: TACACCTACGAACATGACCC;Reverse: TGAAGGTGCGGTCCCATAA |
STAT1 (mus) | Forward: TGGGAAGTATTATTCCAGACCAAA;Reverse: AGTCTTGATGTATCCAGTTCG |
MyD88 (homo) | Forward: CTGGCCTCTGGCATATTC;Reverse: CTCCCTGCTCACATCATTAC |
MyD88 (mus) | Forward: TGCCAGCGAGCTAATTG;Reverse: CACATTCCTTGCTCTGTAGATA |
AKT1 (homo) | Forward: GGCGTGGTCATGTACGA;Reverse: TTCTCATGGTCCTGGTTGTAG |
AKT1 (mus) | Forward: GGACGGGCACATCAAGATAA;Reverse: CCGCAGAATGTCTTCATAG |
AKT2 (homo) | Forward: TACACCTACGAACATGACCC;Reverse: TGAAGGTGCGGTCCCATAA |
AKT2 (mus) | Forward: TTCAGAAGTGGACACAAGGT;Reverse: GGGTCCAGGCTGTCATATC |
IL-1α (homo) | Forward: CGTCAGGCAGAAGTTTGTCA;Reverse: TTAGAGTCGTCTCCTCCCGA |
IL-1α (mus) | Forward: ATCACAGGTAGTGAGACCGA;Reverse: AGCTGATGTGAAGTAGTTCTTAG |
IL-1β (homo) | Forward: CTAAAGTATGGGCTGGACTG;Reverse: AGCTTCAATGAAAGACCTCA |
IL-1β (mus) | Forward: CAAGGAGAACCAAGCAACGA;Reverse: TTTCATTACACAGGACAGGTATAGA |
TNF-α (homo) | Forward: TGTCTACTGAACTTCGGGGT;Reverse: TCACAGAGCAATGACTCCAA |
TNF-α (mus) | Forward: AGGTTCTCTTCAAGGGACAA;Reverse: GACTTTCTCCTGGTATGAGATAG |