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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: From association to mechanism in complex disease genetics: the role of the 3D genome

Fig. 3

Proximity ligation. Chromatin are crosslinked to preserve interactions between proximal regions of DNA and associated proteins. Crosslinked chromatin are digested using restriction enzymes (scissors) to create two short DNA fragments complexed with associated proteins. “Sticky ends” of the two DNA fragments originally in close 3D proximity are then ligated using DNA ligase to create a chimeric strand of DNA. After de-crosslinking, the chimeric DNA can be used in downstream applications to identify and characterize loop formation

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