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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: CCN family member 2/connective tissue growth factor (CCN2/CTGF) is regulated by Wnt–β-catenin signaling in nucleus pulposus cells

Fig. 3

Effect of Wnt–β-catenin signaling on CCN2 expression in rat nucleus pulposus (NP) cells. a Rat NP cells transfected with the CCN2 reporter plasmid together with the pGL4.74 plasmid were treated with different concentrations (0, 0.1, 0.5, 1.0 μM) of 6-bromoindirubin-3′-oxime (BIO) for 24 h. b, c NP cells were cotransfected with the CCN2 reporter plasmid together with WT-β-catenin (b), β-catenin siRNA (si-β-catenin) (c), or empty vectors and the pGL4.74 vector. Cells were cultured for 24 h and luciferase (luc) reporter activity was measured. The results were normalized for transfection efficiency and are expressed as the ratio of luciferase relative to pGL4.74 activities (denoted as relative activity). d NP cells transfected with the CCN2 reporter plasmid were treated with the β-catenin siRNA (si-β-catenin), which was added during exposure of the cells to 1.0 μM BIO. Results are presented as mean and 95% CI (n = ≥ 8 for each group). One-way analysis of variance with the Tukey-Kramer post-hoc test was used for all experiments

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