Fig. 2
From: Downregulation of miRNA17–92 cluster marks Vγ9Vδ2 T cells from patients with rheumatoid arthritis
Production of proinflammatory cytokines by Vγ9Vδ2 T cells. Panels on the left show the gating strategy. a–d Ex-vivo analysis of cytokine-producing Vγ9Vδ2 T cells. a Basal level of cytokines and b after ionomycin-PMA stimulation of one representative rheumatoid arthritis (RA) patient; c basal level of cytokines and d after ionomycin-PMA of one representative healthy donor (HD). e Comparison of proinflammatory cytokines (interferon (IFN)-γ, interleukin (IL)-6, and IL-8) production as a total frequency of cytokine-producing Vγ9Vδ2 T cells between HD (black column) and RA patients (grey column). f,g Ex-vivo analysis of cytokine-producing Vγ9Vδ2 T cells among RA patients and HD (f) and after ionomycin-PMA stimulation (g). The mean percentage of Vγ9Vδ2 T cells expressing IFN-γ, IL-6, or IL-8 among 10 RA patients and 10 HD. **p < 0.01, ***p < 0.001. FSC forward scatter, SSC side scatter