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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Polydatin effectively attenuates disease activity in lupus-prone mouse models by blocking ROS-mediated NET formation

Fig. 4

In vivo, PD prevented spontaneous NET formation in pristane-induced lupus mice through inhibition of ROS. a After eliminating erythrocytes, the remaining cells from the mouse peripheral blood were incubated in RPMI-1640 with 5% fetal bovine serum. The percentage of Ly6G+PI+ cells in mouse peripheral blood was tested by flow cytometry. b Bone marrow-derived neutrophils from mice were cultured in the absence of serum for 4 h and then the spontaneous NET formation measured. c ROS production by the bone marrow-derived neutrophil was measured. For all experiments, data were represented as mean ± SD, #p < 0.05; ##p < 0.01; ###p < 0.001 were for comparisons between vehicle-treated PIL mouse model and vehicle-treated normal mice; *p < 0.05; **p < 0.01; ***p < 0.001 compared the vehicle and PD-treated PIL mice. DAPI 4′, 6′-diamidino-2-phenylindole, MPO myeloperoxidase, NET neutrophil extracellular trap, PD polydatin, PIL pristane-induced lupus

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