Fig. 4

In vivo, PD prevented spontaneous NET formation in pristane-induced lupus mice through inhibition of ROS. a After eliminating erythrocytes, the remaining cells from the mouse peripheral blood were incubated in RPMI-1640 with 5% fetal bovine serum. The percentage of Ly6G⁺PI⁺ cells in mouse peripheral blood was tested by flow cytometry. b Bone marrow-derived neutrophils from mice were cultured in the absence of serum for 4 h and then the spontaneous NET formation measured. c ROS production by the bone marrow-derived neutrophil was measured. For all experiments, data were represented as mean ± SD, ^#p < 0.05; ^##p < 0.01; ^###p < 0.001 were for comparisons between vehicle-treated PIL mouse model and vehicle-treated normal mice; *p < 0.05; **p < 0.01; ***p < 0.001 compared the vehicle and PD-treated PIL mice. DAPI 4′, 6′-diamidino-2-phenylindole, MPO myeloperoxidase, NET neutrophil extracellular trap, PD polydatin, PIL pristane-induced lupus