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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: The effect of the cholinergic anti-inflammatory pathway on collagen-induced arthritis involves the modulation of dendritic cell differentiation

Fig. 5

GTS-21 efficiently inhibited the expression of CD80 and major histocompatibility complex II (MHC II) on the surface of dendritic cells (DCs) during DC differentiation. Bone marrow–derived DCs were stimulated with GTS-21 (0.1, 1, 10, or 100 μmol/L) for 6 days. The expression levels of MHC II and CD80 were detected by using flow cytometry. All data shown were gated on CD11c+ F4/80− cells. a, c, e, g Histograms of MHC II and CD80 in CD11c+ F4/80− cells are shown. b, d, f, h The changes of mean fluorescence intensity (MFI) of MHC II and CD80 were analyzed. (b, d) GTS-21 (1, 10, or 100 μmol/L) decreased the MFI of CD80 and MHC II. (f, h) GTS-21 (10 μmol/L) significantly decreased the MFI of CD80 and MHC II, and methyllycaconitine (MLA) reversed this effect. Data are expressed as the mean ± standard deviation. *P <0.05 versus the control (“ctrl”) group. All data are representative of three independent experiments

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Arthritis Research & Therapy

ISSN: 1478-6362

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