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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: TNFα expressed on the surface of microparticles modulates endothelial cell fate in rheumatoid arthritis

Fig. 2

Gating strategy for flow cytometric analysis. a All microparticles (MPs) were gated as described in “Materials and methods”. b Flow cytometry analysis of specific binding of antibodies to MPs derived from platelets, leukocytes and endothelial cells and annexin-V (AV). c MPs were also labeled for the surface expression of TNFα. SSC, side scatter; FSC, forward scatter; FITC, fluorescein isothiocyanate; PMP, platelet MPs; LMP, leukocyte MPs; EMP, endothelial MPs; APC, allophycocyanin; PE, phycoerythrin; PerCP, peridin chlorophyll protein; T0, time zero (baseline); T4, 4 months after treatment

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