Fig. 2From: TNFα expressed on the surface of microparticles modulates endothelial cell fate in rheumatoid arthritisGating strategy for flow cytometric analysis. a All microparticles (MPs) were gated as described in “Materials and methods”. b Flow cytometry analysis of specific binding of antibodies to MPs derived from platelets, leukocytes and endothelial cells and annexin-V (AV). c MPs were also labeled for the surface expression of TNFα. SSC, side scatter; FSC, forward scatter; FITC, fluorescein isothiocyanate; PMP, platelet MPs; LMP, leukocyte MPs; EMP, endothelial MPs; APC, allophycocyanin; PE, phycoerythrin; PerCP, peridin chlorophyll protein; T0, time zero (baseline); T4, 4 months after treatmentBack to article page