Fig. 6

Altered response of fibroblast-like synoviocytes (FLS) lacking Bma1^−/− to inflammatory stimuli. FLS were cultured from Col6a1-Bmal1^−/− and wild-type littermates to passage 3 and purified for CD90.2 expression before stimulation for 8 h with tumour necrosis factor (TNF)-α. Expression of inflammatory cytokines in cell supernatants was assessed by Bioplex and enzyme-linked immunosorbent assay (CXCL5 only) (n = 5–6). One-way analysis of variance and post hoc Bonferroni correction. Stars directly above TNF-α bars indicate significant difference compared with control counterparts