Fig. 1
Collagen-induced arthritis fibroblast-like synoviocytes (CIA-FLS) modulate T cell migration and serve as antigen-presenting cells in a KCa1.1-dependent manner. a Effector memory T (TEM) cell migration toward CIA-FLS that were cultured for 72 h in the presence or absence of interferon (IFN)-γ, paxilline, or both. Data are presented as mean ± SEM (n = 3). b Flow cytometric images of ovalbumin-loaded, IFN-γ-stimulated CIA-FLS (violet) and ovalbumin-specific TEM cell (green) cocultures that were stained for CD3 (red) after cells were allowed to interact for 30 min. c Flow cytometric plots of mixtures of ovalbumin-loaded, IFN-γ-stimulated CIA-FLS (CellTrace Violet) and ovalbumin-specific TEM cells (carboxyfluorescein succinimidyl ester [CFSE]) that were allowed to interact for 30 min in the presence or absence of paxilline (Pax) or ShK-186. Flow cytometric gating was completed to exclude single cells. d Quantifications of the proportion of CIA-FLS and TEM cells that form conjugates, as defined by the proportion of CellTrace Violet+CFSE+ multiplets or nonsingle cells detected by flow cytometry, from experiments in (c). Data are presented as mean ± SEM (n = 4 separate experiments). e Proliferation of cocultures of CIA-FLS with ovalbumin-specific CD4+ TEM cells in which CIA-FLS were pretreated with IFN-γ, paxilline (Pax), or both and loaded with either ovalbumin (OVA) or myelin basic protein (MBP). Data are presented as mean ± SEM (n = 6). *p < 0.05