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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: KCa1.1 and Kv1.3 channels regulate the interactions between fibroblast-like synoviocytes and T lymphocytes during rheumatoid arthritis

Fig. 2

Collagen-induced arthritis fibroblast-like synoviocytes (CIA-FLS) express major histocompatibility complex (MHC) class II molecules that are regulated through KCa1.1, as well as antigen-presenting proteins. a CIA-FLS plasma membrane expression of MHC class II protein following stimulation for 72 h with IFN-γ (gray circles), paxilline (Pax; black squares), or interferon (IFN)-γ and paxilline (open squares). Data are presented as mean ± SEM (n = 3–8 CIA-FLS donors). b Flow cytometric histogram of CIA-FLS stained for the plasma membrane expression of MHC class II protein in cells treated with paxilline for 72 h (dashed line), stimulated for 72 h with IFN-γ (solid line), or stimulated for 72 h with IFN-γ and paxilline (dotted line). The shaded histogram represents background staining. c MHC class II molecule expression of CIA-FLS that were permeabilized with saponin prior to staining for MHC class II in cells treated for 72 h with IFN-γ (gray circles), paxilline (black squares), or IFN-γ and paxilline (open squares). Data are presented as mean ± SEM (n = 3–6 CIA-FLS donors). d Flow cytometric histogram of CIA-FLS stained for MHC class II molecules following permeabilization with saponin in cells treated for 72 h with IFN-γ (solid line), paxilline (dashed line), or IFN-γ and paxilline (dotted line). The shaded histogram represents background staining. e Expression of intercellular adhesion molecule (ICAM)-1, B7-H3, and CD40 by CIA-FLS following treatment for 72 h with IFN-γ, paxilline, or IFN-γ and paxilline. Data are presented as mean ± SEM (n = 6 CIA-FLS donors). *p < 0.05, **p < 0.01

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