Fig. 3

Microparticles (MPs) and microparticles that form immune complexes (MPs-ICs) from patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) increased the adherence of monocytes to human umbilical vein endothelial cells (HUVEC). Representative pictures of classical (a) and non-classical (b) monocytes adhered to HUVEC treated with MPs and MPs-ICs from patients with RA and SLE; ×40 objective, arrows indicate the endothelial cell and monocytes are in green. c Numbers of classic and non-classical monocytes labeled with carboxyfluorescein succinimidyl ester (CFSE) and adhered to HUVEC or human lung microvascular endothelial cells (HMVEC-L) previously treated with MPs-ICs or MPs from patients with RA and SLE. Data are presented as the mean ± SD. Two-way analysis of variance, *p ≤ 0.05, **p < 0.01, and ***p < 0.001, n = 6, monocytes were counted in at least 5 fields by using a ×40 objective