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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Increased neutrophil extracellular traps activate NLRP3 and inflammatory macrophages in adult-onset Still’s disease

Fig. 2

Enhanced NET release in neutrophils from patients with AOSD. a Neutrophil extracellular trap (NET) release, determined using immunofluorescence microscopy of neutrophils freshly isolated from healthy controls (HC) or adult-onset Still’s disease (AOSD) patients, seeded onto coverslips and treated with 20 nM phorbol myristate acetate (PMA) for 3.5 h. One representative of ten independent experiments is shown. Blue indicates Hoechst; red indicates myeloperoxidase (MPO); green indicates neutrophil elastase (NE). Original magnification 400×, scale bars = 20 μm. b The percentage of NETs (NE, MPO, and Hoechst-labeled neutrophils/total neutrophils) detected in a was quantified. c NET release from heterologous healthy controls (n = 10) or AOSD patients (n = 10) for 3.5 h was quantified using PicoGreen. The histograms show the means ± SD. **P < 0.01, ***P < 0.001

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