Fig. 4

Effect of autophagy inhibition in PBMCs from patients with RA treated with TNFα. a Western blot analysis of LC3-II in PBMCs treated with the autophagy inhibitor 3-MA (10 mM) and TNFα (10 ng/mL) for 24 h. Blot Blot is representative representative of five independent experiments. Densitometry analysis of LC3-II relative to β-actin is also shown, **P < 0.01, *P < 0.05. b Statistical analysis of apoptosis of PBMCs isolated from patients with RA after treatment with 3-MA and TNFα. Results are expressed as AV-positive cells. Representative dot plots (PI on y-axis vs. AV on x-axis) are also shown, **P < 0.01