Fig. 4

KBP and naproxen effects on ankle joint structure and immunohistochemical markers. Tibiotarsal joints were stained using the following procedures: SafO + Fast green staining to assess cartilage damage, TRAP and Mayer’s hematoxylin staining for the detection of osteoclasts, and CD68 and Mayer’s hematoxylin staining for the detection of macrophages. The bone marrow was stained using the following procedures: p75 and Mayer’s hematoxylin staining and TRPV1 and Mayer’s hematoxylin staining, both to assess innervation. Representative micrographs are shown for n = 10/group for the SafO staining and n = 4/group for the other stainings. Scale bars indicate 200 μm