Fig. 1From: Tolerogenic XCR1+ dendritic cell population is dysregulated in HLA-B27 transgenic rat model of spondyloarthritisCD103+CD4− cDCs are depleted in the spleen of B27-Tg rat. Freshly isolated CD103+ splenic cDCs from 6-month-old NTG and B27-Tg rats were analyzed by flow cytometry. a Representative dot-plots showing the expression of CD4 among CD103+ cDCs. b The graph shows the frequency of CD4− and CD4+ cDCs among CD103+ cDCs in NTG and B27-Tg rats. c The graph shows the absolute numbers of CD4− and CD4+ CD103+ cDCs in NTG and B27-Tg rats. d Representative dot-plots showing live (Annexin V−/PI−), apoptotic (Annexin V+/PI−) and dead (Annexin V+/PI+) cell proportions among CD4− splenic CD103+ cDCs. e, f The graphs show the frequency of apoptotic (Annexin V+/PI−) (e) and dead (Annexin V+/PI+) (f) CD4− and CD4+ cDCs in NTG and B27-Tg rats. All experiments were repeated several times, including 8 rats per group. Bars show the mean ± SEM. Data were analyzed by unpaired Student’s t testBack to article page