Fig. 2From: Tolerogenic XCR1+ dendritic cell population is dysregulated in HLA-B27 transgenic rat model of spondyloarthritisThe frequency of XCR1-expressing CD4− cDCs is decreased in the spleen of B27-Tg rat. a–c CD103+cDCs were isolated from the spleen of 6 month-old NTG, B27-Tg, and B7-Tg rats. XCR1 expression was evaluated in CD4− cDCs by flow cytometry (a, b) or by qRT-PCR (c). a Representative dot-plots showing the expression of XCR1 on CD4− CD103+ cDCs in NTG and B27-Tg rats. b The graph shows the frequency of XCR1+ among CD4− CD103+ cDCs in NTG and B27-Tg rats. c The graph shows Xcr1 mRNA levels in purified CD103+CD4− splenic cDCs from NTG, B27-Tg, and B7-Tg rats, expressed in arbitrary units (AU). d–f CD103+cDCs were isolated from the spleen of 1-month-old NTG and B27-Tg rats. d Representative dot-plots showing the expression of XCR1 among CD4− CD103+ cDCs in NTG and B27-Tg rats. e The graph shows the number of XCR1+ cDCs among CD4− CD103+ cDCs in NTG and B27-Tg rats. f The graph shows the XCR1 mean fluorescence intensity (MFI) staining of XCR1+ CD4− cDCs. Experiments were repeated 4–7 times. Bars show the mean ± SEM. Data were analyzed by unpaired Student’s t test (b, e, f) or one-way ANOVA (c)Back to article page