Fig. 3

a, b Decreased capacity of splenic XCR1⁺ CD4⁻ cDCs from B27-Tg rats to support T cell proliferation and Treg differentiation. Sorted XCR1⁺ CD4⁻ CD103⁺ splenic cDCs from NTG (a) and B27-Tg (b) rats were cultured overnight with GM-CSF and tested the next day for their capacity to prime in vitro sorted naïve CD4⁺ CD25⁻ CD62L^high T cells isolated from NTG rat MLN (labeled with CellTrace Violet (CTV) to evaluate T cell proliferation) in the presence of anti-TCRα/β antibody. After 6 days of culture, cells were stained with a live/dead marker, anti-CD4, and anti-FoxP3 antibodies. The plots are gated on live CD4⁺ T cells and show the proliferation of CD4⁺ T cells (as dilution of CellTrace Violet) and FoxP3 expression. Results shown are representative of 2 independent experiments