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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Romidepsin suppresses monosodium urate crystal-induced cytokine production through upregulation of suppressor of cytokine signaling 1 expression

Fig. 2

Suppressive effects of romidepsin on C16.0+MSU-induced cytokine production in human PBMCs. Freshly isolated PBMCs were pre-incubated with different concentrations of romidepsin for 1 h, after which cytokine production was induced via addition of a combination of 50 μM palmitic acid (C16.0) and 300 μg/mL monosodium urate crystals (MSU). The supernatant was collected for extracellular cytokines (ac, f). Cells were lysed with 0.5% Triton X-100, and subsequently, the supernatant of the lysate was collected for measurement intracellular cytokines (de)

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