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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Romidepsin suppresses monosodium urate crystal-induced cytokine production through upregulation of suppressor of cytokine signaling 1 expression

Fig. 3

Effects of romidepsin on cell viability and transcription of IL-1β and NLRP3 inflammasome components. Freshly isolated PBMCs from healthy donors were pre-incubated with different concentrations romidepsin for 1 h, after which a combination of 50 μM palmitic acid (C16.0) and 300 μg/mL monosodium urate crystals (MSU) was added to induce cytokine production. After 24 h of culture, mRNA was measured for IL1B (a), CASP1 (b), NLRP3 (d), and PYCARD (e). Percentages of Annexin V+ and PI+ were measured by flow cytometry to determine cell viability (c, f)

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Arthritis Research & Therapy

ISSN: 1478-6362

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