Fig. 5

Romidepsin induced transcription of SOCS1 and inhibited activation of STAT1 and STAT3. Schematic representations of the SOCS1 and SOCS3 genome and their binding sites for class I HDACs were retrieved from the USCS Genome Browser by means of the track “Transcription Factor ChIP-seq (161 factors) from ENCODE with Factorbook Motifs” (a). PBMCs from healthy volunteers were pre-incubated with romidepsin, after which a combination of 50 μM palmitic acid (C16.0) and 300 μg/mL monosodium urate crystals (MSU) was added. After 24 h, mRNA was isolated and qPCR was performed for SOCS1 and SOCS3 (b, e). Cells were stained extracellularly with CD45-KO and intracellularly with either p-STAT1-PE (c, d) or p-STAT3-PE (f, g) for flow cytometry. Gating on CD45+ lymphocytes and monocytes was performed in the forward versus side scatter graph