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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Romidepsin suppresses monosodium urate crystal-induced cytokine production through upregulation of suppressor of cytokine signaling 1 expression

Fig. 6

Proteasome inhibitor bortezomib reverses cytokine suppression by romidepsin. PBMCs were isolated from healthy volunteers and were pre-incubated for 1 h with bortezomib. Then romidepsin was added for 1 h pre-incubation, after which a combination of 50 μM palmitic acid (C16.0) and 300 μg/mL monosodium urate crystals (MSU) was added for another 24 h. The supernatant was collected for extracellular IL-1β (a). Cells were lysed with 0.5% Triton X-100, and subsequently, the supernatant of the lysate was collected for intracellular IL-1β measurement (b)

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Arthritis Research & Therapy

ISSN: 1478-6362

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