Mass spectrometry-based analysis of cerebrospinal fluid from arthritis patients—immune-related candidate proteins affected by TNF blocking treatment

Estelius, Johanna; Lengqvist, Johan; Ossipova, Elena; Idborg, Helena; Le Maître, Erwan; Andersson, Magnus L. A.; Brundin, Lou; Khademi, Mohsen; Svenungsson, Elisabet; Jakobsson, Per-Johan; Lampa, Jon

doi:10.1186/s13075-019-1846-6

Table 2 Proteins important for separation between patients before and after infliximab treatment

From: Mass spectrometry-based analysis of cerebrospinal fluid from arthritis patients—immune-related candidate proteins affected by TNF blocking treatment

Protein name	Gene symbol	Accession	Univariate analysis			Multivariate analysis		Process	Suggested function(s)
Protein name	Gene symbol	Accession	Fold change^†	Z score	p value	VIP	p (corr)	Process	Suggested function(s)
Cell Adhesion Molecule 3	CADM3	Q8N126	− 0.68	− 1.992	0.046	2.0	0.7	Cell adhesion	Overexpressed in murine microglia after bacterial challenge and may be involved in development of depressive symptoms following immune challenge. [43]
Insulin-like Growth Factor-Binding Protein 7	IGFBP7	Q16270	− 0.50	− 2.201	0.028	1.6	0.7	Cell adhesion	Upregulated in spinal cord during EAE and suggested to be a regulator of oligodendrocyte differentiation. [54]
Protein Tyrosine Phosphatase, Receptor Type N	PTPRN	Q16849	− 0.49	− 2.201	0.028	1.6	0.6	Cell signalling	Important for proper secretion of hormones (insulin) and neurotransmitters [55]
Apolipoprotein H	APOH	P02749	− 0.32	− 1.992	0.046	1.7	0.8	Coagulation	May be associated with brain atrophy in healthy individuals [56]. Is the main antigen in antiphospholipid syndrome and may be associated with CNS related disease in these patients [57]
Fibrinogen gamma chain	FGG	P02679	− 0.61	− 2.201	0.028	1.5	0.5	Immune response, Acute phase protein	Important for proper T cell functioning and neutrophil pathogen clearance [37]. Regulator of microglia activation which may be important in pathogenesis of experimental autoimmune encephalomyelitis [58]
Alpha-1-B Glycoprotein	A1BG	P04217	− 0.39	− 2.201	0.028	2.6	0.7	Immune response, Acute phase protein	–
Beta-2-Microglobulin	B2M	P61769	− 0.44	− 1.992	0.046	1.7	0.8	Immune response, Adaptive immuntity	Increased in circulation in chronic fatigue syndrome [59] and identified as important in CSF of female chronic widespread pain patients [60]. CSF levels of B2M is suggested to reflect immune activation and lymphoid cell turnover in the CNS [61]
Complement C7	C7	P10643	− 0.48	− 2.201	0.028	2.1	0.5	Immune response, Innate immunity	–
Complement Factor B	CFB	P00751	− 0.38	− 1.992	0.046	1.7	0.6	Immune response, Innate immunity	Differentially expressed in AD CSF [62]
Complement C4B (Chido Blood Group)	C4B	P0C0L5	− 0.37	− 2.201	0.028	2.1	0.5	Immune response, Innate immunity	Differentially expressed in CSF of AD patients [62] and elevated in CSF of MS patients with active disease [63]
Hemopexin	HPX	P02790	− 0.33	− 1.992	0.046	1.7	0.7	Oxidative stress protection	Neuroprotective in stroke and intracerebral haemorrhages [64]. Increase in CSF following yeast-induced inflammation [65]

^†Fold change is calculated as “(sample after infliximab − baseline sample)/baseline sample”. Proteins were identified in CSF of polyarthritis patients using label-free proteomics and uni- and multivariate data analysis

Back to article page

ISSN: 1478-6362

Contact us

General enquiries: journalsubmissions@springernature.com

Arthritis Research & Therapy

Contact us