Fig. 5

RvD1 daily treatment prevents cartilage and bone alterations in arthritic mouse joints. Arthritis was induced as previously described. After mouse sacrifice, blood was collected. Knees and ankles were removed and then fixed and sectioned as described in the “Materials and methods” section. Knee sections were stained with safranin-O staining and scored (a). Ankles were examined with a micro-CT scanner (b). Serum RvD1, TRAP, CTX-I, and CTX-II levels were assessed by EIA and ELISA, respectively (c–f). Data are means ± SEM, and one-way ANOVA was performed to compare the results. ^#p < 0.05, ^##p < 0.01, and ^####p < 0.0001 compared to non-immunized group; *p < 0.05, **p < 0.01, and ***p < 0.001 compared to CAIA group. n = 4–5 mice per group. F femur, T tibia